Seminar: Adaptive Focused Acoustics Technology Enabling Multi-Omics Approach in Precision Medicine

When: March 28, 12.30-1.30 PM

Where: 201 Chemistry and Materials Building LSU main campus

The MSF is excited to host Covaris for a seminar on Adaptive Focused Acoustic Technology. which is a novel approach to sample preparation in a variaty of "-omics" fields. Below an introduction from the speaker for this event, Dr. Deb Bhattacharyya. If you are interested in this topic and would like to know more, please click on the RSVP link below. 

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The past decades have witnessed a dramatic improvement of our understanding of the genetic basis of many complex diseases such as, Cancer, Alzheimer’s disease, inherited disease, and many more. From a genomic perspective, the host of technologies and the evolution therein have optimized approaches ensuring reliable extraction, isolation, purification of DNA and RNA for every analytical environment. This ability ultimately enables achievement of high-quality genomic data, regardless of sample complexity or user expertise. However, the need for understanding the behavioral pattern for the entire system pertaining to a specific disease state also created a demand for development of multi-Omics based approach that cover genomics, transcriptomics, epigenomics, proteomics, and metabolomics – the key to advancing precision medicine in the clinic. While the world has witnessed an unprecedented growth of precision medicine workflows, each of the parts of such workflow requires optimal experimental conditions to ensure high quality and confident data from multi-omics analyses. 

Adaptive Focused Acoustics® (AFA®) Technology has been the gold standard for DNA shearing in almost every Next Generation Sequencing (NGS) laboratory and is now gaining unprecedented popularity as the sample preparation technology for a host of Proteomics and protein analysis workflow. The results reported in this presentation will highlight increased reproducibility, reliability, robustness of the method in DNA shearing across different cell lines (S. Cerevisiae, Y. Lipolytica, E.Coli, L. Monocytogenes, Whole Blood, and S. Epidermidis) with very different characteristics. The workflows detailed in this presentation will also highlighting the benefits of a comprehensive workflow that results in high quality and high yield of DNA and RNA from Formalin Fixed Paraffin Embedded (FFPE) tissue samples. The data will show excellent %CV values along with high DV200 scores (for RNA) and high yield and amplifiability for DNA. The presentation will also highlight a one-plate workflow for protein extraction, isolation, digestion, and peptide purification from different biological matrices (fresh frozen tissues, tumor tissues, FFPE, organoids, cell lysates, plasma, etc.) In each example, in addition to a high number of protein ID, the AFA enabled workflows ensure high reproducibility.

Confident data results in accurate prediction – from understanding the behavior of a group of proteins to the Pharmacokinetic and Pharmacodynamic properties of a biotherapeutic monitored in plasma. AFA can enable robust, reliable and confident data for every analyte from any complex biological matrix.